The Power of Decellularization
The proper decellularization of ECMs is critical to the function of the final ECM product. The goal of decellularization is to remove the cells and eliminate residual genetic material, while preserving the ECM components and retaining the properties of the base tissue1. Incomplete decellularization has been shown to decrease the ratio of M2-activated macrophages to M1-macrophages when compared to more complete decellularization methods2. Further studies have demonstrated that higher ratios of M2-activated to M1-activated macrophages are more promotive of immunomodulation, constructive mechanisms, and remodeling activities3.
Watch the effects of extracellular matrix on a stalled wound and the importance of thoroughly decellularizing the ECM.